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goat anti mouse icosl abs  (R&D Systems)


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    R&D Systems goat anti mouse icosl abs
    FIGURE 5. <t>ICOSL</t> is downregulated by ADAM17 on the surface of B cells. (A and B) Flow cytometry analysis of cell surface levels of the indicated costimulatory mole- cules in splenocytes of unimmunized ADAM17+/+ and ADAM17ex/ex mice that were incubated in vitro with one of the fol- lowing: 250 ng/ml PMA, 10 mg/ml anti-IgM F(ab9)2, 1:1 with L or L-ICOS cells for 2 h. (C) Flow cytometry analysis of the cells isolated from the draining LNs and spleens of mice immunized with OVA plus anti- CD40 mAbs and poly(I:C). The percentage of CD40+ or ICOSL+ cells among the gated B cells (B220+) is shown in histograms. Data are from 1 experiment and are repre- sentative of at least 5 experiments (A and B), or from 4–10 experiments, with a horizontal line showing the mean in each group (C). **p , 0.01 by t test.
    Goat Anti Mouse Icosl Abs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+icosl+abs/Mouse+ICOS+Antibody/pm25108021-101-54-58
    Average 90 stars, based on 3 article reviews
    goat anti mouse icosl abs - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses."

    Article Title: The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1302893

    FIGURE 5. ICOSL is downregulated by ADAM17 on the surface of B cells. (A and B) Flow cytometry analysis of cell surface levels of the indicated costimulatory mole- cules in splenocytes of unimmunized ADAM17+/+ and ADAM17ex/ex mice that were incubated in vitro with one of the fol- lowing: 250 ng/ml PMA, 10 mg/ml anti-IgM F(ab9)2, 1:1 with L or L-ICOS cells for 2 h. (C) Flow cytometry analysis of the cells isolated from the draining LNs and spleens of mice immunized with OVA plus anti- CD40 mAbs and poly(I:C). The percentage of CD40+ or ICOSL+ cells among the gated B cells (B220+) is shown in histograms. Data are from 1 experiment and are repre- sentative of at least 5 experiments (A and B), or from 4–10 experiments, with a horizontal line showing the mean in each group (C). **p , 0.01 by t test.
    Figure Legend Snippet: FIGURE 5. ICOSL is downregulated by ADAM17 on the surface of B cells. (A and B) Flow cytometry analysis of cell surface levels of the indicated costimulatory mole- cules in splenocytes of unimmunized ADAM17+/+ and ADAM17ex/ex mice that were incubated in vitro with one of the fol- lowing: 250 ng/ml PMA, 10 mg/ml anti-IgM F(ab9)2, 1:1 with L or L-ICOS cells for 2 h. (C) Flow cytometry analysis of the cells isolated from the draining LNs and spleens of mice immunized with OVA plus anti- CD40 mAbs and poly(I:C). The percentage of CD40+ or ICOSL+ cells among the gated B cells (B220+) is shown in histograms. Data are from 1 experiment and are repre- sentative of at least 5 experiments (A and B), or from 4–10 experiments, with a horizontal line showing the mean in each group (C). **p , 0.01 by t test.

    Techniques Used: Flow Cytometry, Incubation, In Vitro, Isolation

    Related Articles

    Recombinant:

    Article Title: The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.
    Article Snippet: .. One hundred nanograms of recombinant murine ICOSL-IgG fusion protein was incubated with the indicated ratio of recombinant mouse (rm)ADAM17 (R&D Systems) or rhADAM17 in 20 mMHEPES or 50 mM Tris buffer for up to 5 h. The samples were resolved by SDS/6–16% PAGE under reducing conditions and visualized by ECL (BioRad) after incubation with goat anti-mouse ICOSL Abs (R&D Systems), followed by rabbit anti-goat IgG Abs conjugated to HRP (Millipore) or with goat anti-rabbit IgG conjugated to HRP (Sigma-Aldrich). ..

    Incubation:

    Article Title: The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.
    Article Snippet: .. One hundred nanograms of recombinant murine ICOSL-IgG fusion protein was incubated with the indicated ratio of recombinant mouse (rm)ADAM17 (R&D Systems) or rhADAM17 in 20 mMHEPES or 50 mM Tris buffer for up to 5 h. The samples were resolved by SDS/6–16% PAGE under reducing conditions and visualized by ECL (BioRad) after incubation with goat anti-mouse ICOSL Abs (R&D Systems), followed by rabbit anti-goat IgG Abs conjugated to HRP (Millipore) or with goat anti-rabbit IgG conjugated to HRP (Sigma-Aldrich). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.
    Article Snippet: .. One hundred nanograms of recombinant murine ICOSL-IgG fusion protein was incubated with the indicated ratio of recombinant mouse (rm)ADAM17 (R&D Systems) or rhADAM17 in 20 mMHEPES or 50 mM Tris buffer for up to 5 h. The samples were resolved by SDS/6–16% PAGE under reducing conditions and visualized by ECL (BioRad) after incubation with goat anti-mouse ICOSL Abs (R&D Systems), followed by rabbit anti-goat IgG Abs conjugated to HRP (Millipore) or with goat anti-rabbit IgG conjugated to HRP (Sigma-Aldrich). ..



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    goat anti mouse icosl abs  (R&D Systems)
    90
    R&D Systems goat anti mouse icosl abs
    FIGURE 5. <t>ICOSL</t> is downregulated by ADAM17 on the surface of B cells. (A and B) Flow cytometry analysis of cell surface levels of the indicated costimulatory mole- cules in splenocytes of unimmunized ADAM17+/+ and ADAM17ex/ex mice that were incubated in vitro with one of the fol- lowing: 250 ng/ml PMA, 10 mg/ml anti-IgM F(ab9)2, 1:1 with L or L-ICOS cells for 2 h. (C) Flow cytometry analysis of the cells isolated from the draining LNs and spleens of mice immunized with OVA plus anti- CD40 mAbs and poly(I:C). The percentage of CD40+ or ICOSL+ cells among the gated B cells (B220+) is shown in histograms. Data are from 1 experiment and are repre- sentative of at least 5 experiments (A and B), or from 4–10 experiments, with a horizontal line showing the mean in each group (C). **p , 0.01 by t test.
    Goat Anti Mouse Icosl Abs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+icosl+abs/Mouse+ICOS+Antibody/pm25108021-101-54-58
    Average 90 stars, based on 1 article reviews
    goat anti mouse icosl abs - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 5. ICOSL is downregulated by ADAM17 on the surface of B cells. (A and B) Flow cytometry analysis of cell surface levels of the indicated costimulatory mole- cules in splenocytes of unimmunized ADAM17+/+ and ADAM17ex/ex mice that were incubated in vitro with one of the fol- lowing: 250 ng/ml PMA, 10 mg/ml anti-IgM F(ab9)2, 1:1 with L or L-ICOS cells for 2 h. (C) Flow cytometry analysis of the cells isolated from the draining LNs and spleens of mice immunized with OVA plus anti- CD40 mAbs and poly(I:C). The percentage of CD40+ or ICOSL+ cells among the gated B cells (B220+) is shown in histograms. Data are from 1 experiment and are repre- sentative of at least 5 experiments (A and B), or from 4–10 experiments, with a horizontal line showing the mean in each group (C). **p , 0.01 by t test.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: The role of metalloproteinase ADAM17 in regulating ICOS ligand-mediated humoral immune responses.

    doi: 10.4049/jimmunol.1302893

    Figure Lengend Snippet: FIGURE 5. ICOSL is downregulated by ADAM17 on the surface of B cells. (A and B) Flow cytometry analysis of cell surface levels of the indicated costimulatory mole- cules in splenocytes of unimmunized ADAM17+/+ and ADAM17ex/ex mice that were incubated in vitro with one of the fol- lowing: 250 ng/ml PMA, 10 mg/ml anti-IgM F(ab9)2, 1:1 with L or L-ICOS cells for 2 h. (C) Flow cytometry analysis of the cells isolated from the draining LNs and spleens of mice immunized with OVA plus anti- CD40 mAbs and poly(I:C). The percentage of CD40+ or ICOSL+ cells among the gated B cells (B220+) is shown in histograms. Data are from 1 experiment and are repre- sentative of at least 5 experiments (A and B), or from 4–10 experiments, with a horizontal line showing the mean in each group (C). **p , 0.01 by t test.

    Article Snippet: One hundred nanograms of recombinant murine ICOSL-IgG fusion protein was incubated with the indicated ratio of recombinant mouse (rm)ADAM17 (R&D Systems) or rhADAM17 in 20 mMHEPES or 50 mM Tris buffer for up to 5 h. The samples were resolved by SDS/6–16% PAGE under reducing conditions and visualized by ECL (BioRad) after incubation with goat anti-mouse ICOSL Abs (R&D Systems), followed by rabbit anti-goat IgG Abs conjugated to HRP (Millipore) or with goat anti-rabbit IgG conjugated to HRP (Sigma-Aldrich).

    Techniques: Flow Cytometry, Incubation, In Vitro, Isolation